DNA Extraction and Quantification Services for REGARDS Project
NATIONAL INSTITUTES OF HEALTH
Original Solicitation Links
NIH-NOI-PCA-NIA-01601
]regards dna extraction and quantification
Contacts
Description
The Department of Health and Human Services (HHS), National Institutes of Health (NIH), National Institute on Aging (NIA), Center for Alzheimer's and Related Dementias (CARD), intends to negotiate and award a sole source firm-fixed-price contract for specialized laboratory support services for the Reasons for Geographic and Racial Difference in Stroke (REGARDS) project. The contractor will perform DNA extraction, DNA quantification, repository handling, preparation, and shipment of extracted DNA samples for approximately 12,000 REGARDS participant biospecimens to support a genome-wide association study (GWAS). The scope of work includes evaluating frozen packed cell sample availability, selecting and pulling samples from the repository, performing manual DNA extraction using the Qiagen Puregene DNA Purification Kit, quantifying DNA using PicoGreen, creating cryovials of DNA for the REGARDS repository, maintaining tracking inventory for extracted DNA samples, preparing at least 1 ug of DNA per sample at a concentration of 50 ng/uL or greater, shipping extracted DNA samples to CARD facilities, providing DNA quantification results to the Government, and identifying any samples for which extraction failed. The anticipated period of performance is July 1, 2026, through June 30, 2029.
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